CAO Junyan,GE Qingping,LI Jiaojiao
Abstract:
A method was presented for the simultaneous determination of 3 , 6-dichloropicolinic acid ( 3 , 6.DCP) and 3,4,5,6-tetrachloropicolinic acid(3,4,5,6-TCP) in the electrochemieal synthesis reaction liquidby high performance liquid chromatography ( HPLC). The analysis was perfored on a Hypersi C18 columnwith a mixture of V( acetonitrile) : V( volume fraction of 19 acetic acid aqueous solution) = 15 :85 as the mo-bile phase at a flow rate of 1.0ml/min, The samples were detected at 280 nm with an UV detector and thecolumn temperature was 25℃, lt was found that 3 ,6-DCP and 3,4,5,6-TCP could be well separated underthe chromatographic conditions. The maximum relative standard deviations were 0. 58% and 0. 40%6 and thedetection limits were 2.01 x 10~’g/L and 5.09 x 10-’g/L for 3 ,6-DCP and 3 ,4,$ ,6-TCP. The recoveries forthem were 99.88%~100.5%and 99.89%~100.1%,respectively.