Effects of Microsecond Pulsed Electric Field Combined with EPA on the Viability of A-549 Cells

NAVIGATE

Table of Contents

STATISTICS

Viewed1172

Downloads800

Effects of Microsecond Pulsed Electric Field Combined with EPA on the Viability of A-549 Cells

[HTML] PDF下载 (800)

[1]ZHANG Jianhua,YANG Yanjun,LYU Yanpeng,et al.Effects of Microsecond Pulsed Electric Field Combined with EPA on the Viability of A-549 Cells[J].Journal of Zhengzhou University (Engineering Science),2022,43(05):98-103.[doi:10.13705/j.issn.1671-6833.2022.05.012]

Copy

Journal of Zhengzhou University (Engineering Science)[ISSN 1671-6833/CN 41-1339/T] Volume: 43卷 Number of periods: 2022 05 Page number: 98-103 Column: Public date: 2022-08-22

Title:: Effects of Microsecond Pulsed Electric Field Combined with EPA on the Viability of A-549 Cells

Author(s):: ZHANG Jianhua¹; YANG Yanjun¹; LYU Yanpeng¹; JIN Wenwen¹; CHEN Chuanliang²; 1.School of Electrical Engineering, Zhengzhou University, Zhengzhou 450001, China;
2.Department of Medical Imaging, Henan Provincial People′s Hospital, Zhengzhou 450003, China

Keywords:: microsecond pulsed electric field; eicosapentaenoic acid; lung cancer A-549 cells; cell membrane permeability; cell activity

CLC:: N39；TN782

DOI:: 10.13705/j.issn.1671-6833.2022.05.012

Abstract:: In view of the problems that traditional chemotherapeutic drugs used in current electrochemical therapy have large toxic and side effects on human normal tissues, and traditional Chinese medicine ingredients have little toxic and side effects on human body, but have weak effects. Eicosapentaenoic acid (EPA), a monomer component of traditional Chinese medicine, was selected as an anticancer drug to explore the promoting effect of microsecond pulse electric field on the cytotoxic effect of EPA, and the toxic effect of the combination of the two on lung cancer A-549 cells. Firstly, the permeability of cell membrane under the action of pulsed electric field with different electric field strengths, and the effects of different drug concentrations (50—1 000 μmol/L) and electric field strengths (750—2 000 V/cm) on cell activity were studied. PI staining was used to detect cell membrane permeability, and CCK-8 assay was used to detect cell viability. Then, according to the detection results, the electric field intensity range for subsequent experiments was selected to be 1 000—1 500 V/cm, and the EPA concentration range was 100—400 μmol/L. Finally, the electric field combined with the drug group was set as the experimental group, the blank control group, the pure drug group, and the pure electric group were set as the control group. The experimental study on the cytotoxicity of pulsed electric field combined with EPA on A-549 cells was carried out, and the changes of A-549 cell viability at 24 h (48 h) after the combined action of pulsed electric field and EPA were compared and analyzed. Research shows that microsecond pulsed electric field could significantly enhance the toxic effect of EPA on A-549 cells, and when the concentration of EPA was 300 μmol/L, the combined effect of pulsed electric field and EPA was the best. The enhancement effect of microsecond pulsed electric field on EPA cytotoxicity could reach more than 40%, which provided an important promotion method for the subsequent intervention of traditional Chinese medicine ingredients in the treatment of cancer.

References:: [1] DAVALOS R V, MIR L M, RUBINSKY B. Tissue ablation with irreversible electroporation[ J] . Annals of biomedical engineering, 2005, 33(2) : 223-231.
[2] 卢强, 闫小龙, 黄立军, 等. 高压电场不可逆电穿孔诱发 A549 肺癌细胞凋亡的实验研究[ J] . 现代生物医学进展, 2014, 14(3) : 425-428, 556.
LU Q, YAN X L, HUANG L J, et al. Empirical study of A549 lung cancer cells apoptosis induced by irreversible electroporation of high voltage electric field [ J] . Progress in modern biomedicine, 2014, 14(3) : 425-428, 556.
[3] 张涛, 郭一民, 李卓扬, 等. 碳量子点诱导人体肝癌细胞凋亡的研究[ J] . 郑州大学学报( 工学版) , 2020, 41(5) : 1-7.
ZHANG T, GUO Y M, LI Z Y, et al. Carbon quantum dots as potent agent to induce apoptosis of human hepatoma cells [ J ] . Journal of Zhengzhou university ( engineering science) , 2020, 41(5) : 1-7.
[4] WEAVER J C. Electroporation of biological membranes from multicellular to nano scales [ J ] . IEEE transactions on dielectrics and electrical insulation, 2003, 10(5) : 754-768.
[5] MCBRIDE S, AVAZZADEH S, WHEATLEY A M, et al. Ablation modalities for therapeutic intervention in arrhythmia-related cardiovascular disease: focus on electroporation [ J ] . Journal of clinical medicine, 2021, 10(12) : 2657.
[6] MIR L M. Bases and rationale of the electrochemotherapy [ J ] . European journal of cancer supplements, 2006, 4(11) : 38-44.
[7] ZHANG L Z, GAO S G, HE J. The role of maintenance therapy in the treatment of elderly non-small-cell lung cancer patients: a meta-analysis of randomized controlled trials [ J ] . Drug design, development and therapy, 2017, 11: 3435-3440.
[8] 杨曦月, 杜小波. 晚期非小细胞肺癌治疗方案的研究进展及三线治疗探索 [ J] . 保健医学研究与实践, 2021, 18(4) : 137-143.
YANG X Y, DU X B. Research progress of treatment options for advanced non-small cell lung cancer and exploration of third-line treatment [ J ] . Health medicine research and practice, 2021, 18(4) : 137-143.
[9] 玄永哲, 南昶辉, 马刘江, 等. 二十碳五烯酸协同长春瑞滨对人肺癌 A-549 细胞的作用[ J] . 延边大学医学学报, 2012, 35(3) : 210-214.
XUAN Y Z, NAN C H, MA L J, et al. Effects of eicosapentaenoic acid and its synergistic effects with vinorelbine on A-549 human lung cancer cell line [ J] . Journal of medical science Yanbian university, 2012, 35(3) : 210-214.
[10] BERQUIN I M, EDWARDS I J, CHEN Y Q. Multitargeted therapy of cancer by omega-3 fatty acids[ J] . Cancer letters, 2008, 269(2) : 363-377.
[11] OGO A, MIYAKE S, KUBOTA H, et al. The mechanism of the synergistic anticancer effect of CDDP and EPA in the TE1 cell line [ J] . Anticancer research, 2021, 41(4) : 1771-1778.
[12] 姚陈果, 宁郡怡, 刘红梅, 等. 微 / 纳秒脉冲电场靶向不同尺寸肿瘤细胞内外膜电穿孔效应研究[ J] . 电工技术学报, 2020, 35(1) : 115-124.
YAO C G, NING J Y, LIU H M, et al. Study of electroporation effect of different size tumor cells targeted by micro-nanosecond pulsed electric field [ J] . Transactions of China electrotechnical society, 2020, 35 (1) : 115-124.
[13] ZAJDEL A, WILCZOK A, TARKOWSKI M. Toxic effects of n-3 polyunsaturated fatty acids in human lung A549 cells [ J] . Toxicology in vitro, 2015, 30 ( 1) : 486-491.
[14] MORTAZ E, MOLOUDIZARGARI M, KHOSRAVI A, et al. EPA and DHA have selective toxicity for PBMCs from multiple myeloma patients in a partly caspase-dependent manner [ J ] . Clinical nutrition, 2020, 39(7) : 2137-2143.
[15] OONO K, OHTAKE K, WATANABE C, et al. Contribution of Pyk2 pathway and reactive oxygen species (ROS) to the anti-cancer effects of eicosapentaenoic acid ( EPA) in PC3 prostate cancer cells[ J] . Lipids in health and disease, 2020, 19(1) : 15-22.

Similar References:

Memo

Last Update: 2022-08-23