Optimization for Cellulose Carrier Immobilized Glucoamylase by Response Surface Methodology
[1]Chen Junying,Zhou Hangyu,Tang Huanyan,et al.Optimization for Cellulose Carrier Immobilized Glucoamylase by Response Surface Methodology[J].Journal of Zhengzhou University (Engineering Science),2019,40(02):67-74.[doi:10.13705/j.issn.1671-6833.2018.05.016]
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Journal of Zhengzhou University (Engineering Science)[ISSN
1671-6833/CN
41-1339/T] Volume:
40
Number of periods:
2019 02
Page number:
67-74
Column:
Public date:
2019-03-19
- Title:
- Optimization for Cellulose Carrier Immobilized Glucoamylase by Response Surface Methodology
- Keywords:
- cellulose; sodium alginate; Immobilized; Glucoamylase; Response Surface Method
- CLC:
- -
- Abstract:
- Cellulose,nad loose pore and long chain structure are favorable for the permeation and adsorption of macromolecules. When the calcium chloride solution was added to the mixed solution of cellulose and sodium alginate, cellulose and alginate can be cross-linked with calcium ions to form the gel. So glucoamylase could be immobilized by the carrier materials mixed with cellulose and sodium alginate. Some factors which affect the immobilized enzyme activity were considered, such as mix proportion for cellulose and sodium alginate, mass concentration of the mixed solution for cellulose and sodium alginate, and mass concentration of calcium chloride solution. The response surface methodology was carried out based on single factor test, and the optimal conditions for preparing immobilized glucoamylase were obtained as follows: the ratio of cellulose and sodium alginate was 1.5:1, the mass concentration of the mixed solution for cellulose and sodium alginate was 2.80%, the mass concentration of calcium chloride solution was 2.77%. The optimal conditions’ result of response surface methodology was tested successfully. In addition, the reusability test was conducted, and the results showed that the immobilized glucoamylase which adsorbed to the enzyme solution was better than unadsorbed and immobilized glucoamylase.
- References:
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Last Update:
2019-03-24