Optimization of Purification Process of Scutellaria baicalensis Extract by PB Test with Response Surface Methodology

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Optimization of Purification Process of Scutellaria baicalensis Extract by PB Test with Response Surface Methodology

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[1]SUN Xin,WANG Xianli,MA Xiaoji,et al.Optimization of Purification Process of Scutellaria baicalensis Extract by PB Test with Response Surface Methodology[J].Journal of Zhengzhou University (Engineering Science),2023,44(04):107-112,119.[doi:10.13705/j.issn.1671-6833.2023.01.002]

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Journal of Zhengzhou University (Engineering Science)[ISSN 1671-6833/CN 41-1339/T] Volume: 44卷 Number of periods: 2023 04 Page number: 107-112,119 Column: Public date: 2023-06-01

Title:: Optimization of Purification Process of Scutellaria baicalensis Extract by PB Test with Response Surface Methodology

Author(s):: SUN Xin¹; WANG Xianli¹; MA Xiaoji¹; DENG Fangfang²; PAN Chengxue¹; 2; 1.School of Pharmacy, Zhengzhou University, 450001, Zhengzhou, Henan, 2.Henan Zhengda Jienuo Pharmaceutical Technology Co., Ltd., Henan Zhengzhou 450001.

Keywords:: baicalin; purification; Plackett-Burman test; Box-Behnken response surface test; quality by design

CLC:: R284.2

DOI:: 10.13705/j.issn.1671-6833.2023.01.002

Abstract:: Taking the yield and purity of baicalin as evaluation indicators, the acid precipitation method, alkali-soluble acid precipitation method, recrystallization method and pharmacopeia purification method were used to purify baicalin, and the best purification method was determined as alkali-soluble acid precipitation method. Plackett-Burman test was used to obtain the key process parameters in the alkali-soluble acid precipitation method, and the regression model between the key process parameters and the key evaluation indicators was established by the Box-Behnken response surface test, and the design space was obtained and verified. The key parameters obtained by screening were the liquid concentration, alkali solution temperature, alkali solution pH, acid precipitation temperature and acid precipitation pH. When the yield and purity of baicalin were both greater than 85%, the operating space were: the alkali solution temperature was 40 ℃ , the pH of alkali solution was 8. 0, the temperature of acid precipitation was 80 ℃ , the concentration of liquid medicine was 11. 53 ~ 14 mg·mL - 1 , and the pH of acid precipitation was 1. 17 ~ 2. 33. The verification results show that the design space was stable and reliable, and it could provide a reference for industrial production of Scutellaria baicalensis extract.

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Last Update: 2023-07-01