Study on Immobilization of Lipase with Fe3O4 Magnetic Chitiosan Micropheres
[1]Han Xiuli,Gu Pengju,Fang Shuqi,et al.Study on Immobilization of Lipase with Fe3O4 Magnetic Chitiosan Micropheres[J].Journal of Zhengzhou University (Engineering Science),2018,39(04):30-35.[doi:1013705/j.issn.1671-6833.2018.01.014]
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Journal of Zhengzhou University (Engineering Science)[ISSN
1671-6833/CN
41-1339/T] Volume:
39卷
Number of periods:
2018 04
Page number:
30-35
Column:
Public date:
2018-07-22
- Title:
- Study on Immobilization of Lipase with Fe3O4 Magnetic Chitiosan Micropheres
- Keywords:
- Magnetic chitosan microspheres; Azide; lipase
- CLC:
- -
- DOI:
- 1013705/j.issn.1671-6833.2018.01.014
- Abstract:
- In order to increase the utilization rate of lipase, the immobilization of lipase by azidation magnetic chitosan was investigated. The magnetic chitosan microspheres with particle size of 40~60 µm were prepared by suspension croddlinking method. The microspheres were modified by grafting and azide. Response surface methodology was used to investigate the effect of reaction conditions on the immobilized enzyme, and the optimum conditions for lipase immobilization were concentration of enzyme 4 mg/ml, reaction time 8.4h, reaction temperature 39.3ºc and the ph 7.0. In the optimum conditions, the immobilized enzyme amount of magnetic chitosan microsheres was 64.4 mg/g, with the actual value showing a good fit to the predicted value. The results suggested that this method could be uaed to immobilized lipase.
- References:
- -
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Last Update:
2018-07-25