Enzyme-catalyzed photometric determination of peracetic acid content in air disinfectants

Peracetic acid;  Horseradish peroxidase;  online pre-enrichment;  air disinfection;  Enzymatic analysis

When peracetic acid (PAcA) is used as a disinfectant indoors, the content in the air is very low and it is difficult to detect with conventional chemical analysis methods. The content of trace peracetic acid disinfectant in indoor environment was determined by enzyme-catalyzed spectrophotometry by air disinfection by spray method and fumigation method, and on-site sampling with gas absorption tube. Experiments show that PAcA can react with phenol and 4-aminoantipyrine when phosphate buffer is present under the catalysis of horseradish peroxidase (HRP). Similar to the enzyme-catalyzed spectrophotometry of hydrogen peroxide, the quinone dye generated at 35 °C has complete color development for 30 min, has maximum absorption at 505 nm, and the absorbance coefficient of the corresponding wavelength is 12.72L/(mmol·cm), and the peroxide is measured