Circulating Tumour Cell Detection Based on YOLOv11 and Vascular-like Chip
[1]LI Haoliang,QIAO Linbo,LI Jianing,et al.Circulating Tumour Cell Detection Based on YOLOv11 and Vascular-like Chip[J].Journal of Zhengzhou University (Engineering Science),2026,47(XX):1-8.[doi:10.13705/j.issn.1671-6833.2026.04.005]
Copy
Journal of Zhengzhou University (Engineering Science)[ISSN
1671-6833/CN
41-1339/T] Volume:
47
Number of periods:
2026 XX
Page number:
1-8
Column:
Public date:
2026-09-10
- Title:
- Circulating Tumour Cell Detection Based on YOLOv11 and Vascular-like Chip
- CLC:
- TH789 TP391.4 R318.6
- Abstract:
- To address the challenge of accurately identifying low-abundance circulating tumor cell (CTC) in whole blood samples, where interference from abundant normal blood cells complicates detection, a dynamic blood circulation platform was developed. The proposed platform integrated microfluidic vascular-like chips with peristaltic pumps, employing bright-field high-speed imaging and YOLOv11 object detection to enable label-free CTC detection in whole blood. The system simulated blood flow using a peristaltic pump, creating a flowing environment with circulatory characteristics. The chip employed multi-level branching and a 40 μm micro-screen structure, with channel dimensions optimized according to cell and vascular sizes. An image dataset comprising four cell types, including CTC (human colon cancer cell HT29 model), RBC, WBC, and PLT, was constructed. The experimental results showed that the mAP of the system for CTC detection was 98.65%, and the Precision and Recall were both greater than 99%. Continuous frame online detection was demonstrated. The system could simulate in vitro blood circulation and accurately identify CTC in whole blood samples, exhibiting high accuracy and stability. It provided a novel technical approach for early tumor diagnosis.
- References:
-
[1] GOETZ J G. Metastases go with the flow[J]. Science, 2018, 362(6418): 999-1000.
[2] CAO W, CHEN H D, YU Y W, et al. Changing profiles of cancer burden worldwide and in China: a secondary analysis of the global cancer statistics 2020[J]. Chinese Medical Journal, 2021, 134(7): 783-791.
[3] BIAN X L, YIN S J, YANG S, et al. Roles of platelets in tumor invasion and metastasis: a review[J]. Heliyon, 2022, 8(12): e12072.
[4] RING A, NGUYEN-STRÄULI B D, WICKI A, et al. Biology, vulnerabilities and clinical applications of circulating tumour cells[J]. Nature Reviews Cancer, 2023, 23(2): 95-111.
[5] RUPP B, BALL H, WUCHU F, et al. Circulating tumor cells in precision medicine: challenges and opportunities[J]. Trends in Pharmacological Sciences, 2022, 43(5): 378-391.
[6] QIAO Y Y, LIN K X, ZHANG Z, et al. Monitoring disease progression and treatment efficacy with circulating tumor cells in esophageal squamous cell carcinoma: a case report[J]. World Journal of Gastroenterology, 2015, 21(25): 7921-7928.
[7] SMIT D J, PANTEL K. Circulating tumor cells as liquid biopsy markers in cancer patients[J]. Molecular Aspects of Medicine, 2024, 96: 101258.
[8] COSTA C, MUINELO-ROMAY L, CEBEY-LÓPEZ V, et al. Analysis of a real-world cohort of metastatic breast cancer patients shows circulating tumor cell clusters (CTC-clusters) as predictors of patient outcomes[J]. Cancers, 2020, 12(5): 1111.
[9] CARLSSON A, NAIR V S, LUTTGEN M S, et al. Circulating tumor microemboli diagnostics for patients with non-small-cell lung cancer[J]. Journal of Thoracic Oncology, 2014, 9(8): 1111-1119.
[10] XIE N, HU Z Y, TIAN C, et al. In vivo detection of CTC and CTC plakoglobin status helps predict prognosis in patients with metastatic breast cancer[J]. Pathology & Oncology Research, 2020, 26(4): 2435-2442.
[11] ANDREE K C, VAN DALUM G, TERSTAPPEN L W M M. Challenges in circulating tumor cell detection by the CellSearch system[J]. Molecular Oncology, 2016, 10(3): 395-407.
[12] AU S H, EDD J, STODDARD A E, et al. Microfluidic isolation of circulating tumor cell clusters by size and asymmetry[J]. Scientific Reports, 2017, 7(1): 2433.
[13] MATSUMURA H, SHEN L T, ISOZAKI A, et al. Virtual-freezing fluorescence imaging flow cytometry with 5-aminolevulinic acid stimulation and antibody labeling for detecting all forms of circulating tumor cells[J]. Lab on a Chip, 2023, 23(6): 1561-1575.
[14] HABLI Z, ALCHAMAA W, SAAB R, et al. Circulating tumor cell detection technologies and clinical utility: challenges and opportunities[J]. Cancers, 2020, 12(7): 1930.
[15] GALANZHA E I, MENYAEV Y A, YADEM A C, et al. In vivo liquid biopsy using Cytophone platform for photoacoustic detection of circulating tumor cells in patients with melanoma[J]. Science Translational Medicine, 2019, 11(496): eaat5857.
[16] ALVARADO-ESTRADA K, MARENCO-HILLEMBRAND L, MAHARJAN S, et al. Circulatory shear stress induces molecular changes and side population enrichment in primary tumor-derived lung cancer cells with higher metastatic potential[J]. Scientific Reports, 2021, 11: 2800.
[17] LE M N, SMITH K A, ALIPANAH M, et al. Microfluidics-enabled isolation and single-cell analysis of circulating tumor cells[J]. Methods in Molecular Biology, 2023, 2689: 71-93.
[18] EDD J F, MISHRA A, DUBASH T D, et al. Microfluidic concentration and separation of circulating tumor cell clusters from large blood volumes[J]. Lab on a Chip, 2020, 20(3): 558-567.
[19] VORA N, SHEKAR P, HANULIA T, et al. Deep learning-enabled detection of rare circulating tumor cell clusters in whole blood using label-free, flow cytometry[J]. Lab on a Chip, 2024, 24(8): 2237-2252.
[20] PARK J, HA S, KIM J, et al. Classification of circulating tumor cell clusters by morphological characteristics using convolutional neural network-support vector machine[J]. Sensors and Actuators B: Chemical, 2024, 401: 134896.
[21] DU Z Q, LI Y, CHEN B, et al. Label-free detection and enumeration of rare circulating tumor cells by bright-field image cytometry and multi-frame image correlation analysis[J]. Lab on a Chip, 2022, 22(18): 3390-3401.
[22] BOYA M, OZKAYA-AHMADOV T, SWAIN B E, et al. High throughput, label-free isolation of circulating tumor cell clusters in meshed microwells[J]. Nature Communications, 2022, 13: 3385.
[23] PATTANAYAK P, SINGH S K, GULATI M, et al. Microfluidic chips: recent advances, critical strategies in design, applications and future perspectives[J]. Microfluidics and Nanofluidics, 2021, 25(12): 99.
[24] ZHOU J W, ELLIS A V, VOELCKER N H. Recent developments in PDMS surface modification for microfluidic devices[J]. Electrophoresis, 2010, 31(1): 2-16.
[25] 刘长春, 崔大付, 王利. 聚二甲基硅氧烷微流体芯片的制作技术[J]. 传感器技术, 2004, 23(7): 77-80.
LIU C C, CUI D F, WANG L. Fabrication technology of polydimethylsiloxane microfluidic chip[J]. Journal of Transducer Technology, 2004, 23(7): 77-80.
- Similar References:
Memo
-
Last Update:
2026-02-26
