Isolation of lycopene from the crude extract of red watermelon flesh by high-speed counter-current chromatograghy
[1]Ruan Baoyu,Zhao Wenen.Isolation of lycopene from the crude extract of red watermelon flesh by high-speed counter-current chromatograghy[J].Journal of Zhengzhou University (Engineering Science),2016,37(05):43-46.[doi:10.3969/j.issn.1671-6833.2016.05.009]
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Journal of Zhengzhou University (Engineering Science)[ISSN
1671-6833/CN
41-1339/T] Volume:
37
Number of periods:
2016 05
Page number:
43-46
Column:
Public date:
2016-11-25
- Title:
- Isolation of lycopene from the crude extract of red watermelon flesh by high-speed counter-current chromatograghy
- CLC:
- -
- Abstract:
- Lycopene was separated from the crude extract of red watermelon pulp by high-speed countercurrent chromatography (HSCCC). Under the action of ultrasonic waves, v petroleum ether: v acetone = 2:1 was used as the extraction solvent to extract lycopene from 400 g of mature watermelon pulp homogenate Crude lycopene, until all the pigments are extracted. Then use a two-phase solvent system composed of vn-n-hexane:v acetonitrile:v dichloromethane=20:12:7 for preparative high-speed countercurrent chromatography separation, from about 223 mg About 30 mg lycopene was obtained from the crude extract. Calculated by the peak area normalization method, the product purity was 98.3%.
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