[1]WENG Haibo,JING Weiran,YANG Danli,et al.Screening and Characterization of an Thermophilic Alkaline Crude Proteasefrom an Isolated Strain of Bacillus subtilis I 1 5[J].Journal of Zhengzhou University (Engineering Science),2010,31(01):73-.
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Journal of Zhengzhou University (Engineering Science)[ISSN
1671-6833/CN
41-1339/T] Volume:
31卷
Number of periods:
2010 01
Page number:
73-
Column:
Public date:
2010-01-30
- Title:
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Screening and Characterization of an Thermophilic Alkaline Crude Proteasefrom an Isolated Strain of Bacillus subtilis I 1 5
- Author(s):
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WENG Haibo; JING Weiran; YANG Danli; etc
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Department of Bioengineering of Zhengzhou University,Zhengzhou 450001,China
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- Keywords:
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thermophilic alkaline protease; screening; characterization
- CLC:
-
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- DOI:
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- Abstract:
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A strain producing higher activity protease named Bacillus subtilis 115 is isolated and identifiedfrom waste—rich soil which stacked high temperature and long time.We optimize the fermentation conditionsof this strain and study a part of cnzymology properties.The results show that corn starch and soybean cakepowder are the optimization of this strain as carbon and nitrogen source for thermophilic alkaline protease pro—duetion.The protease belongs to thermophilic alkaline protease,which shows high pH stability and thermal stability.In addition,it shows excellent stability and compatibility with surfactant and detergent.These propertiesindicate that the protease has abright market prospect.