Comparison of the Determination Methods for Genistein from theWaste Residue of the Soybeans
[1]LI Hua,LI Dan.Comparison of the Determination Methods for Genistein from theWaste Residue of the Soybeans[J].Journal of Zhengzhou University (Engineering Science),2009,30(04):62-64,102.
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Journal of Zhengzhou University (Engineering Science)[ISSN
1671-6833/CN
41-1339/T] Volume:
30卷
Number of periods:
2009年04期
Page number:
62-64,102
Column:
Public date:
1900-01-01
- Title:
- Comparison of the Determination Methods for Genistein from theWaste Residue of the Soybeans
- Keywords:
- genistein; TLC; UV; HPLC; determination
- CLC:
- -
- DOI:
- -
- Abstract:
- using ethanol—aqueous solution to extract soybean isoflarone from the waste residue of the soybean．After preliminary purification，high—purity genistein was obtained with AB一8 macroporous resin by gradientelution．Samples were determined method and compared with the TLC．UV and HPLC．The results showedthat：The TLC method is simple and the separation is effective；the UV method was high accuracy，high preci—sion and good reproducibility．The relative average deviation for the recovery test was 1．04％，the relative average deviation for the tests of precision was 0．24％and the relative average deviation for the repeatability testwas 0．09％；the HPLC method was high resolution，high sensitivity，etc．The sample purity was 90．1％．
- References:
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Last Update:
1900-01-01