According to the molecular structure characteristics of cyclohexene and the basic theory of related unit reactions, a process route for the preparation of 1,2-cyclohexanediol from cyclohexene oxidation by hydrogen peroxide under the condition of acetic acid and acetic anhydride as oxygen carrier was proposed. The influence of material ratio, reaction temperature, reaction time and other factors was investigated by orthogonal test method and single factor experimental method, and the product was extracted by solvent, and the melting point test and infrared spectrometer analysis method were used to confirm that the product of the synthesis reaction was trans-1,2-cyclohexanediol.