Using polyethersulfone hollow fiber membrane as the support film, a composite charged mosaic film that can effectively transfer electrolyte and retain low molecular weight organic matter was prepared by interfacial polymerization. The uniform design was used for the process of preparing charged mosaic membranes by interfacial polymerization, and the regression equation was obtained by using SPSS software for data processing, which described the influence of various factors on the separation performance of the membrane. In the experimental range, the acid acceptant concentration had little effect on the membrane separation factor; 4-(chloromethyl)benzoyl fluoride concentration and surfactant concentration can be maximumed; There was an interaction between polyethyleneimine concentration and interfacial polymerization reaction time. The calculated values of the model are in good agreement with the experimental results, indicating that the model can be used to guide the practice of film formation. Under the optimized conditions, the interception rate of membrane to inorganic salts does not exceed 30%, the interception rate of membrane to sucrose is greater than 90%, and the interception rate of p-xylenol orange can reach 96%, indicating that the membrane can be used for the separation of inorganic salts and low molecular weight organic matter.