[1]陈晓岚,李慧,袁金伟,等.ESI MS研究黄酮含磷衍生物与溶菌酶的竞争作用[J].郑州大学学报(工学版),2009,30(03):106-105.[doi:10.3969/j.issn.1671-6833.2009.03.027]
　CHEN Xiaolan,LI Hui,Yuan Jinwei,et al.ESI MS studies the competitive effects of flavonoids containing phosphorus-containing derivatives with lysozyme[J].Journal of Zhengzhou University (Engineering Science),2009,30(03):106-105.[doi:10.3969/j.issn.1671-6833.2009.03.027]

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ESI MS研究黄酮含磷衍生物与溶菌酶的竞争作用()

分享到：

《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:

30卷

期数:

2009年03期

页码:

106-105

栏目:

出版日期:

1900-01-01

文章信息/Info

Title:

ESI MS studies the competitive effects of flavonoids containing phosphorus-containing derivatives with lysozyme

作者:

陈晓岚; 李慧; 袁金伟; 等.

郑州大学,化学系,河南省化学生物与有机化学重点实验室,河南,郑州,450001, 中原工学院河南,郑州,450007, 郑州大学,化学系,河南省化学生物与有机化学重点实验室,河南,郑州,450001;河南工业大学,化学化工学院,河南,郑州,450002

Author(s):

CHEN Xiaolan; LI Hui; Yuan Jinwei; etc

关键词:

ESI质谱;  黄酮-7-磷酰化氨基酸酯;  黄酮-7-二异丙基磷酸酯;  溶菌酶;  竞争作用

Keywords:

ESI mass spectrometry;  Flavonoid-7-phosphorylated amino acid esters;  Flavonoid-7-diisopropyl phosphate;  Lysozyme;  Competitive role

DOI:

10.3969/j.issn.1671-6833.2009.03.027

文献标志码:

A

摘要:

采用电喷雾质谱(ESI MS)技术,研究了黄酮-7-磷酰化丙氨酸酯(a)、黄酮-7-磷酰化缬氨酸酯(b)、黄酮-7-磷酰化亮氨酸酯(c)、黄酮-7-磷酰化异亮氨酸酯(d)分别与黄酮-7-二异丙基磷酸酯(e)、溶菌酶形成的三体系混合溶液小分子与蛋白大分子的弱相互作用.实验结果表明,在这个三体系的混合溶液中,黄酮-7-磷酰化氨基酸酯a、b、c、d与黄酮-7-二异丙基磷酸酯e相比,与溶菌酶具有更强的弱相互作用能力或亲和力.

Abstract:

The weak interaction between flavonoid-7-phosphorylated alanine (a), flavonoid-7-phosphorylated valine (b), flavonoid-7-phosphorylated leucine (c), flavonoid-7-phosphorylated leucine (d) and flavonoid-7-phosphorylated isoleucine ester (d) was studied by electrospray mass spectrometry (ESI) with flavonoid-7-diisopropyl phosphate (e) and lysozyme in the three-system mixed solution small molecules and protein macromolecules, respectively. The experimental results show that flavonoid-7-phosphorylated amino acid esters a, b, c, d have stronger weak interaction ability or affinity with lysozyme than flavonoid-<>-diisopropyl phosphate E in the mixed solution of the three systems.

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