[1]孟志芬,董彩霞,孟现超..酶法提取芝麻叶中总黄酮的工艺研究[J].郑州大学学报(工学版),2005,26(04):53-56.[doi:10.3969/j.issn.1671-6833.2005.04.013]
　Meng Zhifen,Dong Caixia,Meng Xianchao.Enzymatic extraction of total flavonoids from sesame leaves[J].Journal of Zhengzhou University (Engineering Science),2005,26(04):53-56.[doi:10.3969/j.issn.1671-6833.2005.04.013]

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酶法提取芝麻叶中总黄酮的工艺研究()

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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:

26卷

期数:

2005年04期

页码:

53-56

栏目:

出版日期:

1900-01-01

文章信息/Info

Title:

Enzymatic extraction of total flavonoids from sesame leaves

作者:

孟志芬; 董彩霞; 孟现超.

河南科技学院化工系,河南,新乡,453003, 河南科技学院化工系,河南,新乡,453003, 河南科技学院化工系,河南,新乡,453003

Author(s):

Meng Zhifen; Dong Caixia; Meng Xianchao

关键词:

酶法;  提取;  芝麻叶;  黄酮类化合物

Keywords:

Enzymatic;  extraction;  Sesame leaves;  Flavonoids

DOI:

10.3969/j.issn.1671-6833.2005.04.013

文献标志码:

A

摘要:

采用纤维素酶酶解预处理与水浸提相结合的提取工艺从芝麻叶中提取总黄酮,用分光光度法测定了芝麻叶中总黄酮的含量,并对芝麻叶总黄酮的提取过程中各因素对收率的影响情况进行了研究,得出最佳工艺条件为:料液比1:60;pH 5.0;酶解温度45℃;酶浓度3 U/mL;酶解时间2.5 h;浸提时间2.5 h;浸提温度80℃;芝麻叶总黄酮提取收率达12.31%.

Abstract:

The extraction process combining cellulase enzymatic hydrolysis pretreatment and water leaching was used to extract total flavonoids from sesame leaves, the content of total flavonoids in sesame leaves was determined by spectrophotometry, and the influence of various factors on the yield during the extraction of total flavonoids in sesame leaves was studied, and the optimal process conditions were as follows: material-liquid ratio was 1:60. pH 5.0; Enzymatic hydrolysis temperature 45 °C; Enzyme concentration 3 U/mL; Enzymatic hydrolysis time 2.5 h; Leaching time 2.5 h; Leaching temperature 80°C; The extraction yield of total flavonoids from sesame leaves reached 12.31%.

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