The simultaneous extraction of oil and protein from soybean by AOT/isooctane system was studied. The experimental results show that the extraction rate of protein is affected by the extraction time, pH value, salt concentration and AOT concentration of the aqueous solution, and the extraction effect of oil is basically unchanged compared with the traditional oil production process. Orthogonal experimental analysis showed that the optimal conditions for the extraction time were 90 min, salt concentration of 0.1 mol/L, pH value of 5.0, and AOT concentration of 6 g/50 ml of isooctane.