[1]陈珍珍,路 培,符 贵,等.小檗碱改性羧甲基纤维素的制备及抑菌性评价[J].郑州大学学报(工学版),2023,44(02):104-110.[doi:10.13705/j.issn.1671-6833.2023.02.010]
　CHEN Zhenzhen,LU Pei,FU Gui,et al.Preparation and Antibacterial Evaluation of Berberine Modified Carboxymethyl Cellulose[J].Journal of Zhengzhou University (Engineering Science),2023,44(02):104-110.[doi:10.13705/j.issn.1671-6833.2023.02.010]
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小檗碱改性羧甲基纤维素的制备及抑菌性评价()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
44卷
期数:
2023年02期
页码:
104-110
栏目:
出版日期:
2023-02-27

文章信息/Info

Title:
Preparation and Antibacterial Evaluation of Berberine Modified Carboxymethyl Cellulose
作者:
陈珍珍;  路 培;  符 贵;  李 华
郑州大学 化工学院,河南 郑州 450001

Author(s):
CHEN Zhenzhen;  LU Pei;  FU Gui;  LI Hua
School of Chemical Engineering, Zhengzhou University, 450001, Zhengzhou, Henan

关键词:
改性羧甲基纤维素;  小檗碱;  响应曲面法;  抑菌剂;  纸张保护
Keywords:
modified carboxymethyl cellulose;  berberine;  response surface methodology;  bacteriostatic agent;  paper protection
分类号:
O629. 3
DOI:
10.13705/j.issn.1671-6833.2023.02.010
文献标志码:
A
摘要:
为了保护纸张并解决细菌耐药性问题,以羧甲基纤维素和小檗碱为原料,通过希夫碱反应制备了小檗碱 化羧甲基纤维素(BBR-DCMC) 。 采用单因素实验和响应面法优化了制备工艺,采用纸片琼脂扩散法进行抑菌性测 定。 研究结果表明:BBR-DCMC 的合成最佳工艺条件为双醛羧甲基纤维素与改性小檗碱质量比为 7 ∶ 15,反应温度 39. 0℃ ,pH 值为 5. 0,且由该响应面法所建立的模型真实可靠。 此外,BBR-DCMC 对细菌和霉菌有较好的抑菌防霉 性,对金黄色葡萄球菌和大肠杆菌的最小抑菌浓度 MIC 均为 0. 2 mg / mL,对黑曲霉和白念珠菌的最小抑菌浓度均 为 0. 4 mg / mL。 该研究为纸质文物的保护提供了一定的理论指导。
Abstract:
In order to protect paper and solve the problem of bacterial drug resistance, in this study, carboxymethyl cellulose and berberine were used as raw materials to prepare berberylated carboxymethyl cellulose (BBR-DCMC) through schiff base reaction. The single factor experiment and response surface method were used to optimize the preparation process, and disk AGAR diffusion method was used to determine the antibacterial activity. The results showed that when the mass ratio of dialdehyde carboxymethyl cellulose and modified berberine was 7 ∶15, reaction temperature was 39. 0 ℃ , and pH was 5. 0, the optimum synthetic conditions was obtained. And the model established by the response surface method was true and reliable. Besides, BBR-DCMC had good antibacterial and antifungal properties against bacteria and molds. The minimum inhibitory concentration of the product against Staphylococcus aureus and Escherichia coli were 0. 2 mg / mL, the minimum inhibitory concentrations against Aspergillus niger and Candida albicans were 0. 4 mg / ml. This research provided some theoretical guidance for the protection of paper cultural relics.

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更新日期/Last Update: 2023-02-25