[1]张涛,郭一民,李卓扬,等.碳量子点诱导人体肝癌细胞凋亡的研究[J].郑州大学学报(工学版),2020,41(05):1-7.
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碳量子点诱导人体肝癌细胞凋亡的研究()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
41
期数:
2020年05期
页码:
1-7
栏目:
出版日期:
2020-10-01

文章信息/Info

Title:
Carbon Quantum Dots as Potent Agent to Induce A poptosis of Human Hepatoma Cells
作者:
张涛郭一民李卓扬席果果左 磊曹永平王雅泓
文献标志码:
A
摘要:
采用石墨脉冲电解法制备了碳量子点溶胶 ,利用透射电子显微镜、X射线衍射仪、傅里叶变换红外光谱和荧光光谱等手段对碳量子点的形状、尺寸、结构和性能进行了表征。碳量子点为近似球形,平均粒径为6.93nm,表面含有多种富氧官能团并具有荧光特性。体外细胞毒性试验表明:当碳量子点浓度高于一定值时,溶胶能诱导人体肝癌细胞凋亡,对其具有显著的抑制作用。对碳量子点诱导凋亡后的人体肝癌细胞进行了透射电子显微镜观察,结果显示其对线粒体产生了损伤。在相同的浓度范围内,碳量子点溶胶对人体淋巴细胞几乎没有产生影响。碳量子点溶胶具有选择性诱导人体肝癌细胞凋亡的作用,对于研究新型无毒副作用的抗癌药物具有重要意义。
Abstract:
The carbon quantum dots ( CQDs) colloid solution was prepared by electrolyzing graphite with pulsecurrent. The shape, size, structure and performance of the carbon quantum dots were characterized by trans-mission electron microscopy, X -ray diffractometer, Fourier transform infrared spectroscopy and fluorescencespectroscopy , etc. The CQDs were quasi-spherical particles with an average particle size of 6.93 nm, and thesurface contained a variety of oxygen-rich functional groups and showed fluorescence characteristics. The cyto-toxicity test showed that the colloid solution could induce the apoptosis of human hepatoma cells ( HepG2cells) , and thus could effectively inhibit the proliferation of the cells, when the concentration of CQDs colloidsolution was above a certain value. Transmission electron microscopy images of HepG2 cells after apoptosis in-duced by CQDs showed that their mitochondria were damaged. There was almost no effects on human lympho-cytes in the same CQDs concentration range. The CQDs colloid solution has the effect of selectively inducingapoptosis of HepG2 cells, which is of great significance for the research of new anti-cancer drugs without sideeffects.
更新日期/Last Update: 2020-10-23