[1]翁海波,敬蔚然,杨丹丽,等.产高温碱性蛋白酶菌株筛选及酶学性质研究[J].郑州大学学报(工学版),2010,31(01):73.[doi:10.3969/j.issn.1671-6833.2010.01.017]
Weng Haibo,Respectfully,Yang Danli,et al.Screening and enzymatic properties of high-temperature alkaline protease strains[J].Journal of Zhengzhou University (Engineering Science),2010,31(01):73.[doi:10.3969/j.issn.1671-6833.2010.01.017]
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产高温碱性蛋白酶菌株筛选及酶学性质研究(
)
《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]
- 卷:
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31卷
- 期数:
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2010年01期
- 页码:
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73
- 栏目:
-
- 出版日期:
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2010-01-30
文章信息/Info
- Title:
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Screening and enzymatic properties of high-temperature alkaline protease strains
- 作者:
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翁海波; 敬蔚然; 杨丹丽; 等.
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郑州大学生物工程系,郑州,河南,450001
- Author(s):
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Weng Haibo; Respectfully; Yang Danli; etc
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-
- DOI:
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10.3969/j.issn.1671-6833.2010.01.017
- 文献标志码:
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A
- 摘要:
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从高温堆肥土样中筛选到一株高产蛋白酶的菌株,经鉴定为Bacillus subtilis,命名为I15.对最适产酶发酵条件进行了优化,并研究了发酵产蛋白酶的酶学性质.结果表明,玉米淀粉和豆饼粉是该菌发酵产蛋白酶的最适经济碳氮源;该蛋白酶属高温碱性蛋白酶,具有很强的热稳定性和酸碱稳定性.另外,突出的性质是该蛋白酶对表面活性剂和温和型去垢剂具有超强的耐受性,将具有广阔的市场前景.
- Abstract:
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A strain with high protease production was identified as Bacillus subtilis and named I15 from high-temperature compost soil samples. The results showed that corn starch and soybean cake flour were the most suitable and economical sources of carbon and nitrogen for protease production by fermentation. This protease is a high-temperature alkaline protease with strong thermal stability and acid-base stability. In addition, the outstanding property is that the protease has super resistance to surfactants and mild detergents, which will have broad market prospects.
更新日期/Last Update:
1900-01-01