Small-leaved lilac (Syringa pubescens Turcz.) It is a plant of the genus Synnga of the family Oleaceae, in which the main medicinal active ingredient is olive bitter glycoside belonging to the splitting cyclic ether terpene glycosides. The leaves, stems and buds of small-leaf cloves were selected as explants, callus induction and culture were carried out on MS and LS media, and the cell growth cycle of suspension culture was determined after three successions, and the suspension cell culture system of small-leaf clove was established for the first time. The content of oleuropein in suspension cell culture and culture medium was determined by RP-HPLC method, and its structure was determined by 3HNMR and 1CNMR.