[1]张建华,杨延钧,吕彦鹏,等.微秒脉冲可逆电穿孔联合EPA对肺癌A-549细胞活性的影响[J].郑州大学学报(工学版),2022,43(05):98-103.[doi:10.13705/j.issn.1671-6833.2022.05.012]
　ZHANG Jianhua,YANG Yanjun,LYU Yanpeng,et al.Effects of Microsecond Pulsed Electric Field Combined with EPA on the Viability of A-549 Cells[J].Journal of Zhengzhou University (Engineering Science),2022,43(05):98-103.[doi:10.13705/j.issn.1671-6833.2022.05.012]
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微秒脉冲可逆电穿孔联合EPA对肺癌A-549细胞活性的影响()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
43卷
期数:
2022年05期
页码:
98-103
栏目:
出版日期:
2022-08-22

文章信息/Info

Title:
Effects of Microsecond Pulsed Electric Field Combined with EPA on the Viability of A-549 Cells
作者:
张建华1 ;  杨延钧1 ;  吕彦鹏1 ;  金雯雯1 ;  陈传亮2
1.郑州大学电气工程学院;2.河南省人民医院放射科;

Author(s):
ZHANG Jianhua1;  YANG Yanjun1;  LYU Yanpeng1;  JIN Wenwen1;  CHEN Chuanliang2
1.School of Electrical Engineering, Zhengzhou University, Zhengzhou 450001, China; 
2.Department of Medical Imaging, Henan Provincial People′s Hospital, Zhengzhou 450003, China
关键词:
Keywords:
microsecond pulsed electric field;  eicosapentaenoic acid;  lung cancer A-549 cells;  cell membrane permeability;  cell activity
分类号:
N39；TN782
DOI:
10.13705/j.issn.1671-6833.2022.05.012
文献标志码:
A
摘要:
针对当前电化学疗法中使用的传统化疗药物对人体正常组织毒副作用较大,中药成分对人体毒副作用小但作用效果弱等问题,选用中药单体成分二十碳五烯酸( EPA)作为抗癌药物,探究微秒脉冲电场对 EPA 细胞毒性效果的促进作用,以及两者联合作用对肺癌 A-549 细胞的 毒性 效果。 首先,研究不同场强 的脉 冲电场作用下细胞膜通透性变化情况,以及不同的药物浓度( 50 ~ 1 000 μmol / L) 、电场强度( 750 ~ 2 000 V / cm)对 A-549 细胞活性的影响,采用 PI 染色法检 测细胞 膜通 透性、CCK-8 法检测细胞活 性;其次,根据检测结果筛选用于后续实验的电场强度区间为 1 000 ~ 1 500 V / cm,EPA 浓度为 100 ~ 400 μmol / L;最后,设置电场联合药物组为实验组,空白对照组、纯药物组、纯电组为对照组,进 行了脉 冲电场联合 EPA 对 A-549 细胞毒性效果的实验研究,并且对脉冲电场联合 EPA 共同作用后培养 24 h(48 h)时A-549 细胞活性的变化情况进行了 对比分析。 研究表明:微秒脉冲电场能够显著增强 EPA 对 A-549 细胞的毒性效果,并且 EPA 浓度为 300 μmol / L 时,脉冲电场和 EPA 的联合作用效果最佳。 微秒脉冲电场对EPA 细 胞毒性的增强效 果可达 40%以上, 为后续中药成分干 预癌症的治疗提 供了重要的促进手段。
Abstract:
In view of the problems that traditional chemotherapeutic drugs used in current electrochemical therapy have large toxic and side effects on human normal tissues, and traditional Chinese medicine ingredients have little toxic and side effects on human body, but have weak effects. Eicosapentaenoic acid (EPA), a monomer component of traditional Chinese medicine, was selected as an anticancer drug to explore the promoting effect of microsecond pulse electric field on the cytotoxic effect of EPA, and the toxic effect of the combination of the two on lung cancer A-549 cells. Firstly, the permeability of cell membrane under the action of pulsed electric field with different electric field strengths, and the effects of different drug concentrations (50—1 000 μmol/L) and electric field strengths (750—2 000 V/cm) on cell activity were studied. PI staining was used to detect cell membrane permeability, and CCK-8 assay was used to detect cell viability. Then, according to the detection results, the electric field intensity range for subsequent experiments was selected to be 1 000—1 500 V/cm, and the EPA concentration range was 100—400 μmol/L. Finally, the electric field combined with the drug group was set as the experimental group, the blank control group, the pure drug group, and the pure electric group were set as the control group. The experimental study on the cytotoxicity of pulsed electric field combined with EPA on A-549 cells was carried out, and the changes of A-549 cell viability at 24 h (48 h) after the combined action of pulsed electric field and EPA were compared and analyzed. Research shows that microsecond pulsed electric field could significantly enhance the toxic effect of EPA on A-549 cells, and when the concentration of EPA was 300 μmol/L, the combined effect of pulsed electric field and EPA was the best. The enhancement effect of microsecond pulsed electric field on EPA cytotoxicity could reach more than 40%, which provided an important promotion method for the subsequent intervention of traditional Chinese medicine ingredients in the treatment of cancer.

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更新日期/Last Update: 2022-08-23