Under the conditions of nitrogen removal, the operation mode of denitrification SBR was selected, and corresponding enhanced nitrogen removal measures were taken to study the effect of biological denitrification of SBR in monosodium glutamate wastewater. The results show that restrictive aeration is adopted in the water influent stage. The operating conditions were 8 h of influent aeration, 1 h of anaerobic stirring, 1 h of aeration in the back section, 1 h of precipitation and 0.5 h of drainage. The pH value of the nitrification reaction process should be controlled at about 8; The concentration of dissolved oxygen in the nitrification stage and denitrification stage should be controlled at about 2.0 mg/L and 0.5 mg/L, respectively. NH3-N sludge load should be controlled at 0.01~0.02 kg/(kg MLSS·d).When the NH3-N concentration of influent is 18.2~269.1 mg/L, the effluent concentration is 8.0~38.4 mg/L, the removal rate range of NH3-N is 51.1%~87.7%, and the effluent NH3-N index can meet the limit value of 19431 mg/L in GB2004-50< monosodium glutamate industrial pollutant emission standard >.