[1]翁海波,敬蔚然,杨丹丽,等.产高温碱性蛋白酶菌株筛选及酶学性质研究[J].郑州大学学报(工学版),2010,31(01):73.
　WENG Haibo,JING Weiran,YANG Danli,et al.Screening and Characterization of an Thermophilic Alkaline Crude Proteasefrom an Isolated Strain of Bacillus subtilis I 1 5[J].Journal of Zhengzhou University (Engineering Science),2010,31(01):73.
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产高温碱性蛋白酶菌株筛选及酶学性质研究()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
31
期数:
2010年01期
页码:
73
栏目:
出版日期:
2010-01-30

文章信息/Info

Title:
Screening and Characterization of an Thermophilic Alkaline Crude Proteasefrom an Isolated Strain of Bacillus subtilis I 1 5
作者:
翁海波; 敬蔚然; 杨丹丽; 等.
郑州大学生物工程系,郑州,河南,450001
Author(s):
WENG Haibo; JING Weiran; YANG Danli; etc
Department of Bioengineering of Zhengzhou University，Zhengzhou 450001，China
Keywords:
thermophilic alkaline protease; screening; characterization
文献标志码:
A
摘要:
从高温堆肥土样中筛选到一株高产蛋白酶的菌株,经鉴定为Bacillus subtilis,命名为I15.对最适产酶发酵条件进行了优化,并研究了发酵产蛋白酶的酶学性质.结果表明,玉米淀粉和豆饼粉是该菌发酵产蛋白酶的最适经济碳氮源;该蛋白酶属高温碱性蛋白酶,具有很强的热稳定性和酸碱稳定性.另外,突出的性质是该蛋白酶对表面活性剂和温和型去垢剂具有超强的耐受性,将具有广阔的市场前景.
Abstract:
A strain producing higher activity protease named Bacillus subtilis 115 is isolated and identifiedfrom waste—rich soil which stacked high temperature and long time．We optimize the fermentation conditionsof this strain and study a part of cnzymology properties．The results show that corn starch and soybean cakepowder are the optimization of this strain as carbon and nitrogen source for thermophilic alkaline protease pro—duetion．The protease belongs to thermophilic alkaline protease，which shows high pH stability and thermal stability．In addition，it shows excellent stability and compatibility with surfactant and detergent．These propertiesindicate that the protease has abright market prospect．
更新日期/Last Update: 1900-01-01