[1]郭夏丽,杨小丽,李顺义,等.秸秆降解菌的筛选及菌种组合[J].郑州大学学报(工学版),2010,31(01):77.
GUO Xiali,YANG Xiaoli,LI Shunyi,et al.The Screening of Straw--decomposing Microorganism and Strain Combination[J].Journal of Zhengzhou University (Engineering Science),2010,31(01):77.
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秸秆降解菌的筛选及菌种组合()
《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]
- 卷:
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31
- 期数:
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2010年01期
- 页码:
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77
- 栏目:
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- 出版日期:
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2010-01-30
文章信息/Info
- Title:
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The Screening of Straw--decomposing Microorganism and Strain Combination
- 作者:
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郭夏丽; 杨小丽; 李顺义; 等.
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郑州大学化学工程学院,河南,郑州,450001
- Author(s):
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GUO Xiali; YANG Xiaoli; LI Shunyi; etc
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Shool of Chemical Engineering and Energy,Zhengzhou University.Zhengzhou 450001,China
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- Keywords:
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straw returning to farmland; cellulose degradation; screening; strain combination
- 文献标志码:
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A
- 摘要:
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秸秆直接还田会给作物生长带来不利影响,在还田过程中补加纤维素降解菌荆是解决其不利影响的有效方法.通过平板初筛及酶活综合测定及秸秆降解率测定复筛,从腐烂秸秆、牛粪堆肥、森林土等样品中筛选到5株高效纤维素降解菌,其中X1菌的酶活最佳,酶系组成最合理,其Cx,Cb,C1和FPA分别达到53.6 U/mL、69.7 U/mL、9.5 U/mL和11.6 U/mL,对秸秆的降解率达40.2%.进一步进行菌种组合,得到了一组秸秆降解率有明显增加的复合菌X1+X2,其降解率达到45.5%.根据X1和X2的菌落及个体形态特征,初步判定其均为青霉菌.
- Abstract:
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Straw returning to farmland directly has negative effects on seed germination and crop growth.Adding high—efficient cellulose—decomposing bacteria is an effective method to resolve this problem.Fivestrains with different cellulase composition were isolated from rotten straw,COW dung compost,and paddy soil.One of the five strains which named X1 had the maximum cellulage activity and the optimal cellulase composi.tion.In this study the Cx,Cb,C1and FPA of X1 were 53.6 U/mL、69.7 U/mL、9.5 U/mL and 11.6 U/mL,and the straw degradation efficiency was up to 40.20%.The degradation efficiency of the combination ofstrains X1 and X2 Wag up to 45.50%,which higher than the others combination strains.According to themorphological characteristics of X1 and X2,it was deduced that they were Penicillium.
更新日期/Last Update:
1900-01-01