[1]李华,李丹..大豆废料中金雀异黄素含量测定方法的比较[J].郑州大学学报(工学版),2009,30(04):62-64,102.
　LI Hua,LI Dan.Comparison of the Determination Methods for Genistein from theWaste Residue of the Soybeans[J].Journal of Zhengzhou University (Engineering Science),2009,30(04):62-64,102.
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大豆废料中金雀异黄素含量测定方法的比较()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
30
期数:
2009年04期
页码:
62-64,102
栏目:
出版日期:
1900-01-01

文章信息/Info

Title:
Comparison of the Determination Methods for Genistein from theWaste Residue of the Soybeans
作者:
李华; 李丹.
郑州大学化工与能源学院,河南郑州,450001, 郑州大学化工与能源学院,河南郑州,450001
Author(s):
LI Hua; LI Dan
School of Chemical and Energy Engineering，Zhengzhou University，Zhengzhou 450001，China
关键词:
大豆异黄酮;  薄层色谱法;  紫外分光光度法;  高效液相色谱法;  测定
Keywords:
genistein; TLC; UV; HPLC; determination
文献标志码:
A
摘要:
采用乙醇-水溶液从大豆废料中提取大豆异黄酮,初步纯化后,用AB-8大孔吸附树脂进行梯度洗脱得到纯度较高的金雀异黄素样液.样品通过薄层色谱法、紫外分光光度法和高效液相色谱法3种方法测定并做了比较.结果表明:薄层色谱法方法简单,分离效果好;通过紫外分光光度法绘制了标准曲线,可计算样品浓度.该方法准确度高,精密度高,重现性好.加标回收率实验的相对平均偏差RSD为1.04%,精密度实验的相对平均偏差为0.24%,重复性实验的相对平均偏差为0.09%;高效液相色谱法分辨率高、灵敏度高等优点,测得样品纯度为90.1%
Abstract:
using ethanol—aqueous solution to extract soybean isoflarone from the waste residue of the soybean．After preliminary purification，high—purity genistein was obtained with AB一8 macroporous resin by gradientelution．Samples were determined method and compared with the TLC．UV and HPLC．The results showedthat：The TLC method is simple and the separation is effective；the UV method was high accuracy，high preci—sion and good reproducibility．The relative average deviation for the recovery test was 1．04％，the relative average deviation for the tests of precision was 0．24％and the relative average deviation for the repeatability testwas 0．09％；the HPLC method was high resolution，high sensitivity，etc．The sample purity was 90．1％．
更新日期/Last Update: 1900-01-01