川芎嗪衍生物改性丝素材料体外抗凝血性研究-《郑州大学学报(工学版)》

文章信息/Info

Title:: Study on Anticoagulation of Chuanxiongzine Derivative Modified Silk Material in Vitro

作者:: 连小洁; 王松; 朱鹤孙.; 北京理工大学,材料科学与工程学院,北京,100081, 北京理工大学,材料科学与工程学院,北京,100081;北京理工大学,材料科学研究中心,北京,100081, 北京理工大学,材料科学研究中心,北京,100081

Author(s):: LIAN Xiaojie; WANG Song; ZHU Hesun; 1．School ofChemistry and Chemical Engineering，Jiangsu University，Zhenjiang 212013。China；2．School ofMa；erial Scienceand Engineering，Jiangsu University，Zhenjiang 212013，China

Keywords:: silk fibroin; Chinese herbal medicine; Tetramethylpyrazine; Anticoagulant material

摘要:: 为了提高丝素材料的抗凝血性能,尝试用活血化淤中草药川芎的有效成分川芎嗪对其进行改性.首先对川芎嗪的侧链进行结构修饰引入活性基团羟基,然后通过酯化反应引入双键,将得到的中间体进一步在无引发剂条件下接枝聚合在丝素材料表面.用傅立叶转换红外光谱(FTIR)、傅里叶变换衰减全反射红外光谱(ATR-FTIR)、扫描电镜(SEM)对改性丝素进行了结构鉴定.改性丝素的抗凝血性能通过部分凝血活酶时间(APTT)和血小板粘附等实验进行评价,和未改性丝素相比,改性后的丝素APTT时间延长,同时在富血小板血浆中培养30 min后未发现有粘附的血小板,说明川芎嗪衍生物改性丝素材料的体外抗凝血活性有所改善.

Abstract:: In order to improve the anticoagulant properties of silk fibrous material, it was tried to modify it with Chuanxiong, the active ingredient of the Chinese herbal medicine Chuanxiong. Firstly, the side chain of Chuanxiongzine was structurally modified to introduce the active group hydroxyl, and then the double bond was introduced by esterification reaction, and the obtained intermediate was further grafted and polymerized on the surface of silk fibrous material without initiator. The structure of modified silk was identified by Fourier transform infrared spectroscopy (FTIR), Fourier transform attenuated total reflection infrared spectroscopy (ATR-FTIR), and scanning electron microscopy (SEM). The anticoagulant performance of modified silk pigment was evaluated by partial thromboplastin time (APTT) and platelet adhesion, and compared with unmodified silk pigment, the APTT time of modified silk fibroin was prolonged, and no adhesion platelets were found after 30 minutes of culture in platelet-rich plasma, indicating that the anticoagulant activity of Chuanxiongzine derivative modified silk material in vitro was improved.