[1]常春,马晓建,王娟,等.野油菜黄单孢菌的诱变育种及发酵培养基的确定[J].郑州大学学报(工学版),2003,24(01):51-53,57.[doi:10.3969/j.issn.1671-6833.2003.01.011]
　Ivy,MA Xiaojian,WANG Juan,et al.Mutagenesis breeding of Monosporus ferrugina and determination of fermentation medium[J].Journal of Zhengzhou University (Engineering Science),2003,24(01):51-53,57.[doi:10.3969/j.issn.1671-6833.2003.01.011]

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野油菜黄单孢菌的诱变育种及发酵培养基的确定()

分享到：

《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:

24

期数:

2003年01期

页码:

51-53,57

栏目:

出版日期:

1900-01-01

文章信息/Info

Title:

Mutagenesis breeding of Monosporus ferrugina and determination of fermentation medium

作者:

常春; 马晓建; 王娟; 等.

郑州大学化工学院,河南,郑州,450002, 郑州大学化工学院,河南,郑州,450002, 郑州大学化工学院,河南,郑州,450002, 郑州大学化工学院,河南,郑州,450002, 郑州大学化工学院,河南,郑州,450002

Author(s):

Ivy; MA Xiaojian; WANG Juan; etc

关键词:

黄原胶;  野油菜黄单孢黄;  诱发

Keywords:

xanthan gum;  Wild rape yellow monosporus yellow;  Induced

DOI:

10.3969/j.issn.1671-6833.2003.01.011

文献标志码:

A

摘要:

利用紫外线和氯化锂对野油菜黄单孢菌进行诱变,从诱变菌株中筛选出了1株具有较高粘度和产胶率的菌株ZG-1.同时,利用此菌株通过正交试验,确定了发酵培养基配方:淀粉2%,蔗糖2%,蛋白胨0.3%,豆饼粉0.2%,CaCO3,0.3%,柠檬酸0.1%.试验结果表明:菌株的最终发酵液粘度和产胶率由初始的4.5 Pa*s和1.99%提高到12.28 Pa*s和3.45%.

Abstract:

Ultraviolet light and lithium chloride were used to mutagenesis of Monosporus fernosporus, and one strain with high viscosity and gum yield ZG-1 was screened from the mutagenic strains.At the same time, the formula of fermentation medium was determined by orthogonal tests using this strain: starch 1%, sucrose 2%, peptone 2.0%, soybean cake flour 3.0%, CaCO2, 3.0%, citric acid 3.0%.The test results showed that the viscosity and gum yield of the final fermentation broth of the strain increased from 1.4 Pa*s and 5.1% Increased to 99.12 Pa*s and 28.3%.

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